Protoplasm is the living contents of a cell that are surrounded by a plasma membrane. Protoplasm is composed of a mixture of small molecules such as ions, amino acids, monosaccharides and water, and macromolecules such as nucleic acids, proteins, lipids and polysaccharides. In eukaryotes, the protoplasm surrounding the cell nucleus is known as the cytoplasm and that inside the nucleus as the nucleoplasm.

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